Raman spectroscopic characterisation of non stimulated and stimulated human whole saliva

Human saliva is a unique biofluid which can reflect the physiopathological state of an individual. The wide spectrum molecules present in saliva, compounded by close association salivary composition to serum metabolites, provide valuable information for clinical diagnostic applications through highly sensitive vibrational spectroscopic techniques such as Raman spectroscopy. However, nature terms collection and patient-related characteristics, be considered factors may strongly affect spectral profile samples disrupt search specific biomarkers detection diseases. main objective this study was highlight features associated with type intra- inter-patient approach. Saliva collected using both stimulated non-stimulated approaches from 20 donors, concentrated centrifugal filtration further analysed methodology adopted liquid showed consistency qualitative analysis groups, confirming reproducibility Using principal component (PCA) partial least squares – discriminant (PLSDA), non could differentiated analysis, classification efficiency 77 87 %, respectively. bicinchoninic acid (BCA) assay similar trend total protein concentration, showing slight increase samples. These results are process developing establishing spectroscopy novel tool future well controlling variability, order determine markers related multifactorial disease or follow-up purposes.